Correlation of Bone Marrow Morphology and Immunophenotyping in Acute Leukaemia Patients


  • Muhammad Nurul Farhad Dhaka Medical College
  • Salma Afrose BIRDEM, Dhaka
  • Md. Sirajul Islam Sir Salimullah Medical College, Dhaka
  • Gazi Yeasinul Islam Dhaka Medical College Hospital



Leukaemia, bone marrow study, immunophenotyping, correlation


Background: Acute leukaemia (AL) is a malignant disorder of the blood that is characterized by blocked or impaired differentiation of haemopoietic stem cells, resulting in abnormal accumulation of immature precursors and suppression of growth and maturation of cells in vivo. Objective: To find out correlation between morphological and immunophenotypic study of bone marrow among acute leukaemia patient. Methods/Procedure: This is a comparative cross sectional  study of diagnosis of leukaemia by bone marrow study and immunophenotyping from bone marrow sample with bone marrow alone of suspected cases of leukaemia treated in the department of haematology in Dhaka Medical College Hospital (DMCH)from March 2013 to August 2013 .Bone marrow examination and immunophenotyping was done simultaneously but having bone marrow morphology report we have compared with flow report. Results: Out of 50 patients according to Bone marrow study (BMS) 25(50.0%) of the patients had acute myelogenous leukaemia, 24(48.0%) had acute lymphoblastic leukaemia and 1(2.0%) had acute leukaemia. On the other hand, in immunophenotyping 28(56.0%) patients had acute lymphoblastic leukaemia, 20(40.0%) had acute myelogenous leukaemia and 2(4.0%) mixed cell immunophenotyping. Discordance of diagnosis was found in 3(6%) is diagnose as AML which was ALL on flow and one acute leukaemia and one AML was subsequently diagnose as mixed cellular leukaemia. Conclusion: Subjective variation in the accuracy of diagnosis of leukaemia on the basis of bone marrow study alone may occur. Inclusion of immunophenotyping with bone marrow study improves accuracy of leukaemia.

Author Biographies

Muhammad Nurul Farhad, Dhaka Medical College

Assistant Professor, Department of Haematology & BMT, Dhaka Medical College Hospital, Dhaka, Bangladesh

Salma Afrose, BIRDEM, Dhaka

Professor of Haematology, BIRDEM, Dhaka, Bangladesh

Md. Sirajul Islam, Sir Salimullah Medical College, Dhaka

Professor of Hematology, SSMC& Mitford Hospital, Dhaka, Bangladesh

Gazi Yeasinul Islam, Dhaka Medical College Hospital

Department of Haematology & BMT, Dhaka Medical College Hospital, Dhaka, Bangladesh


Rebecca L. Shattuck-Brandt. Stephen J. Brandt, Molecular Biology and Haematology. John P. Greer, John Foerster, George M. Rodgers, et al: Wintrobe’s Clinical Hematology,12th edition: Lippincott Williams & Wilkins, 2009. p-76.

Deschler B, Lubbert M. Acute myeloid leukaemia: epidemiology and etiology. Cancer 2006; 107: 2099–2107.

Ries LAG, Melbert D, Krapcho M, et al. SEER cancer statistics review, 1975–2004, National Cancer Institute. Bethesda, MD. http://seer.cancer. gov/csr/1975_2004, based on November 2006 SEER data submission, posted to the SEER Web site, 2007. 2006.

Liesveld JL, Lichtman MA. Acute Myelogenous Leukaemia; in Marshall A. Lichtman, Ernest Beutler, Thomas J. Kipps, Uri Seligsohn, Kenneth Kaushansky, Josef T. Prchal, Williams Haematology, seventh edition, McGraw-Hill, New Delhi 2006, p-1183.

Rahman M. M, Khan M. A et al. Bone Marrow Examinations: Findings from Four Year Retrospective Single Centre Review J Medicine 2010; 11: 115

Hur M, Chang YH, Lee DS, Park MH, Cho HI. Immunophenotypic and cytogenetic changes in acute leukaemia at relapse. Clin Lab Haematol 2001; 23 (3): 173-9.




How to Cite

Farhad MN, Afrose S, Islam MS, Islam GY. Correlation of Bone Marrow Morphology and Immunophenotyping in Acute Leukaemia Patients. Haematol J Bangladesh [Internet]. 2020Dec.31 [cited 2021Feb.26];4(02):30-2. Available from:



Original Article(s)

Most read articles by the same author(s)

Similar Articles

You may also start an advanced similarity search for this article.