Frequency of Discordance of Diagnosis of Lymphoma by Histopathology alone versus Combined Histopathology and Immunohistochemistry

Mohammed Ali (1) , A K M Mynul Islam (2) , Salina Haque (3) , Md. Kamrul Hasan (4) , Md. Hafizur Rahman (5) , Touhid Uddin Rupom (6) , Md. Abdul Aziz (7) , Md. Mahbubur Rahman (8)
(1) , Bangladesh
(2) , Bangladesh
(3) , Bangladesh
(4) , Bangladesh
(5) , Bangladesh
(6) , Bangladesh
(7) , Bangladesh
(8) , Bangladesh

Abstract

Background: Diagnosis of lymphoma is a critical issue in Bangladesh and still we have to rely on histopathology reports as limited number of centres doing immunohistochemistry (IHC) with panel of incomplete markers. Previously in most of the cases of lymphomas, IHC was not done due to financial constraint and lack of facilities. But IHC is essential for categorization according to WHO classification 2008, through which we can optimize appropriate therapy and predict outcome. Objective: To evaluate the discordance of diagnosis of lymphoma by combined histopathology and immunohistochemistry, and histopathology alone. Methodology: This is a comparative cross-sectional study of diagnoses of lymphoma by immunohistochemistry (IHC) in combination with histopathology of paraffin embedded tissue blocks with histopathology alone of suspected cases of lymphoma treated in the department of Haematology of National Institute of Cancer Research and Hospital (NICR&H) from May 2016 to November 2016. Preliminary histopathological examination was done on tissue biopsy in suspected case of lymphoma and subsequently IHC was done to know the discordance of initial diagnosis with final diagnosis after combined histopathology and IHC. Results: Out of 68 patients 57 (83.8%) were diagnosed as lymphoma on the basis of histopathology alone, among which 41 (60.3%) cases were diagnosed as Non-Hodgkin Lymphoma (NHL) and 16 (23.5%) cases as Hodgkin Lymphoma (HL).After combined histopathology and IHC 61 (89.7%) cases were confirmed as lymphoma of which 50 (73.5%0 were NHL and 11 (16.2%) were HL. Discordance of diagnosis was found in 18 (27%) cases between the two methods of diagnosis. Conclusion: Subjective variation in the accuracy of diagnosis of lymphoma on the basis of histopathology alone may occur. Inclusion of IHC with histopathology improves the accuracy of diagnosis of lymphoma.

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Authors

Mohammed Ali
dr.md.ali383@gmail.com (Primary Contact)
A K M Mynul Islam
Salina Haque
Md. Kamrul Hasan
Md. Hafizur Rahman
Touhid Uddin Rupom
Md. Abdul Aziz
Md. Mahbubur Rahman
Author Biographies

Mohammed Ali

Assistant Professor, Department of Haematology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka

A K M Mynul Islam

Assistant Professor, Department of Haematology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka

Salina Haque

Medical Officer, Department of Haematology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka

Md. Kamrul Hasan

Assistant Professor of Haematology, Colonel Malek Medical College, Manikganj

Md. Hafizur Rahman

Manager, Clinical Haematology and Clinical Microscopy, icddr,b, Mohakhali, Dhaka

Touhid Uddin Rupom

Assistant Professor of Pathology, Shaheed Taj Uddin Ahmed Medical College & Hospital, Gazipur, Dhaka

Md. Abdul Aziz

Associate Professor, Department of Haematology, BSMMU, Shahbag, Dhaka

Md. Mahbubur Rahman

Professor of Haematology, National Institute of Cancer Research and Hospital, Mohakhali, Dhaka

1.
Ali M, Islam AKMM, Haque S, Hasan MK, Rahman MH, Rupom TU, et al. Frequency of Discordance of Diagnosis of Lymphoma by Histopathology alone versus Combined Histopathology and Immunohistochemistry. Haematol J Bangladesh [Internet]. 2020 May 10 [cited 2024 Mar. 28];1(1):2-4. Available from: https://journal.hematologybd.org/index.php/haematoljbd/article/view/4

Article Details

How to Cite

1.
Ali M, Islam AKMM, Haque S, Hasan MK, Rahman MH, Rupom TU, et al. Frequency of Discordance of Diagnosis of Lymphoma by Histopathology alone versus Combined Histopathology and Immunohistochemistry. Haematol J Bangladesh [Internet]. 2020 May 10 [cited 2024 Mar. 28];1(1):2-4. Available from: https://journal.hematologybd.org/index.php/haematoljbd/article/view/4