Expression of Aberrant Immunophenotype Markers in Acute Myeloid Leukaemia in a Tertiary Care Hospital

Papri Nasrin(1), Tania Tasnova(2), Nazia Sharmin(3), Md. Raiq Raihan Chowdhury(4), Maruf Reza Kabir(5), Aminur Rahman(6), Sharup Chandra Poddar(7), Ismat Ara Islam(8), Nasrin Akhter(9), Mily Dey(10), Md. Abdul Aziz(11), Md. Salahuddin Shah(12)
(1) Sir Salimullah Medical College, Dhaka, Bangladesh
(2) Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
(3) Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
(4) Shahid Syed Nazrul Islam Medical College, Kishoreganj, Bangladesh
(5) Dhaka Medical College, Dhaka, Bangladesh
(6) Sheikh Hasina Medical College, Tangail, Bangladesh
(7) Directorate General of Health Services, Dhaka, Bangladesh
(8) Directorate General of Health Service, Dhaka, Bangladesh
(9) Dhaka Medical College, Dhaka, Bangladesh
(10) Sir Salimullah Medical College, Dhaka, Bangladesh
(11) Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
(12) Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

Abstract

Background: Detection of aberrancies in newly diagnosed Acute Myeloid Leukaemia (AML) patients by flow cytometry is of diagnostic and prognostic importance. This study assessed the frequency of expression of aberrant Immunophenotype in newly diagnosed AML patients. Objectives: To assess the frequency of expression of aberrant immunophenotype in patients with acute myeloid leukaemia. Method: This observational cross-sectional study was conducted in the Department of Haematology, BSMMU, Dhaka, for 12 months duration following ethical approval. Total 100 newly diagnosed AML patients were analysed by multiparametric flow cytometry using a panel of monoclonal antibodies. Antigen expression was rated as positive when the percentage of positive blast cells was ?20%. Result: Aberrant lymphoid markers were found in 56% AML cases, wherein 26% had B-cell markers only, 23% had T-cell markers only and 7% had presence of both T-cell and B-cell markers. CD7 (23%) was the most common aberrant marker, followed in decreasing order CD10 (22%), CD19 (11%), CD4 (6%), CD2 (4%) and CD20 (1%). Conclusion: More than half of AML patients had aberrant lymphoid phenotypes. Though aberrance of B-cell markers and T-cell markers are almost same, CD7 was the most common aberrant immunophenotype expressed in AML patients. Further study can be undertaken to correlate prognostic and therapeutic response with these aberrancies.

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References

Aparna SK, & Sharmila M. Aberrant phenotypes in acute myeloid leukaemia in India. International Journal of Advances in Medicine. 2018; 5(2): 361-365

Jahedi M, Shamsasenjan K, Sanaat Z, Aliparasti M, Almasi S, Mohamadian M, Nejati B, Kamalifar A, Movassaghpour AA. Aberrant phenotype in Iranian patients with acute myeloid leukemia. Advanced pharmaceutical bulletin. 2014 Mar;4(1):43.

Azad AK, Khan MR, Habib ABMH, Begum M. Uncommon CD markers in acute myeloid leukaemia. Bangabandhu Sheikh Mujib Med Univ J. 2018; 11:267-269.

Sarma A, Hazarika M, Das D, Kumar Rai A, Sharma JD, Bhuyan C, Kataki AC. Expression of aberrant CD markers in acute leukemia: A study of 100 cases with immunophenotyping by multiparameter flowcytometry. Cancer Biomarkers. 2015 Jan 1;15(4):501-5.

Al-Anizi W. & Al-Mashta M. The frequency of aberrant lymphoid antigens expression in 202 Iraqi patients with de novo acute myeloid leukaemia. Iraqi Journal of Hematology. 2017;6(2):49–54.

Khalidi HS, Medeiros LJ, Chang KL, Brynes RK, Slovak ML & Arber DA. The immunophenotype of adult acute myeloid leukaemia: High frequency of lymphoid antigen expression and comparison of immunophenotype, French- American-British classification, and karyotypic abnormalities. American Journal of Clinical Pathology. 1998; 109(2):211.

Charles NJ, Boyer DF. Mixed-phenotype acute leukemia: diagnostic criteria and pitfalls. Archives of pathology & laboratory medicine. 2017 Nov 1;141(11):1462-8.

Abdulateef NAB, Ismail MM, & Aljedani H. Clinical Significance of Co-expression of Aberrant Antigens in Acute Leukaemia: A Retrospective Cohort Study in Makah Al Mukaramah, Saudi Arabia. Asian Pacific Journal of Cancer Prevention.2014;15(1):221–227.

Shahni A, Saud M, Siddiqui S & Mukry, SN. Expression of aberrant antigens in haematological malignancies: A single center experience. Pakistan journal of medical sciences. 2018;34(2): 457–462.

Ahuja S, & Malviya A. Spectrum of immunophenotypic aberrancies in acute leukaemia along with their correlation with adverse haematological parameters. Indian Journal of Health Sciences and Biomedical Research (KLEU).2022;15(1): 76–80.

Authors

Papri Nasrin
dr.paprinasrin2022@gmail.com (Primary Contact)
Tania Tasnova
Nazia Sharmin
Md. Raiq Raihan Chowdhury
Maruf Reza Kabir
Aminur Rahman
Sharup Chandra Poddar
Ismat Ara Islam
Nasrin Akhter
Mily Dey
Md. Abdul Aziz
Md. Salahuddin Shah
Author Biographies

Papri Nasrin, Sir Salimullah Medical College, Dhaka

Department of Haematology, Sir Salimullah Medical College & Mitford Hospital, Dhaka

Tania Tasnova, Bangabandhu Sheikh Mujib Medical University, Dhaka

Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Dhaka

Nazia Sharmin, Bangabandhu Sheikh Mujib Medical University, Dhaka

Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Dhaka

Md. Raiq Raihan Chowdhury, Shahid Syed Nazrul Islam Medical College, Kishoreganj

Department of Medicine, Shahid Syed Nazrul Islam Medical College, Kishoreganj

Maruf Reza Kabir, Dhaka Medical College, Dhaka

Department of Paediatric haemato-oncology, Dhaka Medical College, Dhaka

Aminur Rahman, Sheikh Hasina Medical College, Tangail

Department of Medicine, Sheikh Hasina Medical College & Hospital, Tangail

Sharup Chandra Poddar, Directorate General of Health Services, Dhaka

Director General of Health Service, Mohakhali, Dhaka

Ismat Ara Islam, Directorate General of Health Service, Dhaka

Medical Officer, Upozilla Health Complex, Gopalpur, Tangail

Nasrin Akhter, Dhaka Medical College, Dhaka

Department of Haematology, Dhaka Medical College, Dhaka

Mily Dey, Sir Salimullah Medical College, Dhaka

Medical Officer, Sir Salimullah Medical College, Mitford, Dhaka

Md. Abdul Aziz, Bangabandhu Sheikh Mujib Medical University, Dhaka

Professor of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka

Md. Salahuddin Shah, Bangabandhu Sheikh Mujib Medical University, Dhaka

Professor and Chairman, Department of Haematology, Bangabandhu Sheikh Mujib Medical University, Shahbag, Dhaka

1.
Nasrin P, Tasnova T, Sharmin N, Chowdhury MRR, Kabir MR, Rahman A, et al. Expression of Aberrant Immunophenotype Markers in Acute Myeloid Leukaemia in a Tertiary Care Hospital. Haematol J Bangladesh [Internet]. 2023 Dec. 31 [cited 2024 Apr. 17];7(2):68-73. Available from: https://journal.hematologybd.org/index.php/haematoljbd/article/view/108

Article Details

How to Cite

1.
Nasrin P, Tasnova T, Sharmin N, Chowdhury MRR, Kabir MR, Rahman A, et al. Expression of Aberrant Immunophenotype Markers in Acute Myeloid Leukaemia in a Tertiary Care Hospital. Haematol J Bangladesh [Internet]. 2023 Dec. 31 [cited 2024 Apr. 17];7(2):68-73. Available from: https://journal.hematologybd.org/index.php/haematoljbd/article/view/108

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