Prevalence of Paroxysmal Nocturnal Haemoglobinuria Clone in Aplastic Anaemia: A Single Centre Study

A. Q. M. Ashraful Haque (1) , Salmun Nahar (2) , A. H. M. Aslam-Ul- Haque (3) , Md. Arif-Ur- Rahman (4) , A B M  Yunus (5) , Tanjina Afrin (6) , Md. Abdul  Aziz (7) , Md. Rafiquzzaman Khan (8) , Md. Salahuddin  Shah (9) , Nusrat Jahan (10)
(1) Colonel Malek Medical College, Manikganj , Bangladesh
(2) Directorate General of Health Services (DGHS), Dhaka , Bangladesh
(3) Dhaka Medical College and Hospital , Bangladesh
(4) , Bangladesh
(5) Bangabandhu Sheikh Mujib Medical University , Bangladesh
(6) Northern International Medical College, Dhaka,Northern International Medical College, Dhaka , Bangladesh
(7) Bangabandhu Sheikh Mujib Medical University , Bangladesh
(8) Bangabandhu Sheikh Mujib Medical University , Bangladesh
(9) Bangabandhu Sheikh Mujib Medical University , Bangladesh
(10) Rupganj Upazila Health Complex, Narayanganj , Bangladesh

Abstract

Background: Flow cytometry assay for PNH clone is a compulsory routine test for all aplastic anaemia patients. Objective: To estimate the frequency of PNH clone in aplastic anaemia. Method: Twenty-two known cases of aplastic anaemia patients were enrolled for the study. Flow cytometric quantitation of glycosyl phosphatidyl-inositol (GPI)-anchored proteins deficiency using markers CD14, CD24, CD45, CD59, Fluorescent Aerolysin (FLAER), CD235a (6 markers) were performed. Result: PNH clone was identified in 8 (36.4%) of the study population. Among PNH clone positive patients 7 (87.5%) were suffering from non-severe aplastic anaemia. Conclusion: PNH clone is significantly associated with aplastic anaemia and PNH clone assay should be regularly assayed in aplastic anaemia patients for specific management. 

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References

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Authors

A. Q. M. Ashraful Haque
draqmhq@gmail.com (Primary Contact)
Salmun Nahar
A. H. M. Aslam-Ul- Haque
Md. Arif-Ur- Rahman
A B M  Yunus
Tanjina Afrin
Md. Abdul  Aziz
Md. Rafiquzzaman Khan
Md. Salahuddin  Shah
Nusrat Jahan
Author Biographies

A. Q. M. Ashraful Haque, Colonel Malek Medical College, Manikganj

Resident Physician (Medicine), Colonel Malek Medical College, Manikganj

Salmun Nahar, Directorate General of Health Services (DGHS), Dhaka

Deputy Program Manager, Directorate General of Health Services (DGHS), Dhaka

A. H. M. Aslam-Ul- Haque, Dhaka Medical College and Hospital

Department of Medicine, Dhaka Medical College Hospital, Dhaka

Md. Arif-Ur- Rahman

Medical Officer, Department of Haematology,Dhaka Medical College Hospital, Dhaka

A B M  Yunus, Bangabandhu Sheikh Mujib Medical University

Professor,Department  of  Haematology,BSMMU, Dhaka, Bangladesh. Email: 

Tanjina Afrin, Northern International Medical College, Dhaka,Northern International Medical College, Dhaka

Assistant Professor, Department  of  Pathology, Northern International Medical College, Dhaka, Bangladesh

Md. Abdul  Aziz, Bangabandhu Sheikh Mujib Medical University

Professsor, Department of Haematology, BSMMU, Dhaka. Bangladesh

Md. Rafiquzzaman Khan, Bangabandhu Sheikh Mujib Medical University

Associate Professor, Department of Haematology, BSMMU, Shahbag, Dhaka

Md. Salahuddin  Shah, Bangabandhu Sheikh Mujib Medical University

Professor of Haematology, BSMMU, Dhaka, Bangladesh

Nusrat Jahan, Rupganj Upazila Health Complex, Narayanganj

Medical Officer, Rupganj Upazila Health Complex, Narayanganj

1.
Haque AQMA, Nahar S, Haque AHMA-U-, Rahman MA-U-, Yunus ABM, Afrin T, et al. Prevalence of Paroxysmal Nocturnal Haemoglobinuria Clone in Aplastic Anaemia: A Single Centre Study. Haematol J Bangladesh [Internet]. 2024 Feb. 14 [cited 2024 Mar. 4];7(1):18-25. Available from: https://journal.hematologybd.org/index.php/haematoljbd/article/view/100

Article Details

How to Cite

1.
Haque AQMA, Nahar S, Haque AHMA-U-, Rahman MA-U-, Yunus ABM, Afrin T, et al. Prevalence of Paroxysmal Nocturnal Haemoglobinuria Clone in Aplastic Anaemia: A Single Centre Study. Haematol J Bangladesh [Internet]. 2024 Feb. 14 [cited 2024 Mar. 4];7(1):18-25. Available from: https://journal.hematologybd.org/index.php/haematoljbd/article/view/100